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KMID : 0895420030130030198
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Journal of Korean Society of Occupational and Enviromental Hygiene
2003 Volume.13 No. 3 p.198 ~ p.203
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Association between Vitamin D Receptor Gene Polymorphisms and Lead Exposure Indices in Lead Workers
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Jang Bong-Ki
Jeong Hyun-Jeong
Lee Jong-Wha
Ahn Kyu-Dong
Lee Byung-Kook
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Abstract
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This study was designed to analyze the relationship between VDR (Vitamin D receptor) genotype and lead exposure indices. VDR genotype of lead workers was analyzed with using BsmI and ApaI restriction enzymes. Also, levels of lead and zinc protoporphyrin (ZPP) in blood, δ-aminolevulinic acid in urine (ALAU), δ-aminolevulinic acid in serum (ALAP), hemoglobin (Hb) and hematocrit (Hct) were measured.
The results were as follows:
1. The frequency of genotype according to BsmI in VDR gene was 0.5 % in BB, 10.2% in Bb, 89.2% in bb. And the frequency of genotype according to ApaI in VDR gene was 18.3% in AA, 25.8% in Aa, 55.9% in aa.
2. The mean of lead in blood of each genotype according to BsmI restriction enzyme was 41.27 ㎍/㎗ in BB?Bb and 34.01 ㎍/㎗ in bb, respectively. Levels of lead in blood of BB?Bb genotype was significantly higher than those of bb genotype (p<0.05). However, there was no significant difference between other lead exposure indices and genotypes.
3. There was no significant difference between each genotype shown because of ApaI restriction enzyme and lead exposure indices. However, the mean of ZPP (84.87㎍/ ㎗) in aa genotype was relatively higher than that (71.02㎍/ ㎗) in AA?Aa.
4. The combination of each VDR genotype gaining from using BsmI and ApaI restriction enzyme resulted in 53.8% in bbaa, 22.0% in bbAa, 13.4% in bbAA, 6.5% in BbAa, 2.2% in Bbaa, 1.6% in BbAA and 0.5% in BBAa.
5. In result to make a comparison between lead exposure indices according to combine genotype, lead workers with the VDR BbAa allele had significantly higher levels of lead in blood (p<0.05) and ALAP (p<0.01) Also, lead workers with the VDR BbAa allele were high ZPP, ALAU, Hb and Hct, but there was not significant difference.
6. Levels of lead in blood and in bone was significantly high as the period of work got longer (p<0.01). It was shown that levels of lead in blood was related with levels of ALAU, ALAP, Hb, Hct. From the above results, VDR genotype according to BsmI and ApaI restriction enzyme, respectively is related to levels of lead in blood. Combined genotype VDR according to BsmI and ApaI polymorphisms is related to levels of lead in blood and ALAP. However, it is considered that many more studies should be necessary to figure out whether VDR genotype according to restriction enzymes and its combined forms
affect the susceptibility of lead and the relationship between two things.
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KEYWORD
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Vitamin D receptor gene, VDR, Polymorphism, Bsml, Apal, Lead exposure indices, Lead workers
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